Characterization of trans-spliced chimeric RNAs: insights into the mechanism of trans-splicing.

ABSTRACT: Trans-splicing is a post-transcriptional processing event that joins exons from separate RNAs to produce a chimeric RNA. However, the detailed mechanism of trans-splicing remains poorly understood. Here, we characterize trans-spliced genes and provide insights into the mechanism of trans-splicing in the tunicate Ciona. Tunicates are the closest invertebrates to humans, and their genes frequently undergo trans-splicing. Our analysis revealed that, in genes that give rise to both trans-spliced and non-trans-spliced messenger RNAs, trans-splice acceptor sites were preferentially located at the first functional acceptor site, and their paired donor sites were weak in both Ciona and humans. Additionally, we found that Cion

SUBMITTER: Yokomori R 

PROVIDER: S-EPMC11155486 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature