CAMSAP2 and CAMSAP3 localize at microtubule intersections to regulate the spatial distribution of microtubules.

ABSTRACT: Microtubule networks support many cellular processes and exhibit a highly ordered architecture. However, due to the limited axial resolution of conventional light microscopy, the structural features of these networks cannot be resolved in three-dimensional (3D) space. Here, we used customized ultra-high-resolution interferometric single-molecule localization microscopy to characterize the microtubule networks in Caco2 cells. We found that the calmodulin-regulated spectrin-associated proteins (CAMSAPs) localize at a portion of microtubule intersections. Further investigation showed that depletion of CAMSAP2 and CAMSAP3 leads to the narrowing of the inter-microtubule distance. Mechanistically, CAMSAPs recognize microtubule defects, which often occur near microtubule intersections, and then r

SUBMITTER: Zhang R 

PROVIDER: S-EPMC11156519 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature