Structural basis of promoter recognition by Staphylococcus aureus RNA polymerase.

ABSTRACT: Bacterial RNAP needs to form holoenzyme with σ factors to initiate transcription. While Staphylococcus aureus σ^A controls housekeeping functions, S. aureus σ^B regulates virulence, biofilm formation, persistence, cell internalization, membrane transport, and antimicrobial resistance. Besides the sequence difference, the spacers between the -35 element and -10 element of σ^B regulated promoters are shorter than those of σ^A regulated promoters. Therefore, how σ^B recognizes and initiates transcription from target promoters can not be inferred from that of the well studied σ. Here, we report the cryo-EM structures of S. aureus RNAP-promoter open complexes comprising σ^A and σ^B, respectively. Structural analyses, in combi

SUBMITTER: Yuan L 

PROVIDER: S-EPMC11156646 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature