Prevalence of ST1049-KL5 carbapenem-resistant Klebsiella pneumoniae with a bla_KPC-2 and bla_NDM-1 co-carrying hypertransmissible IncM1 plasmid.

ABSTRACT: Infection caused by KPC and NDM carbapenemases co-producing Klebsiella pneumoniae (KPC_NDM_CRKP) poses serious public health concerns. Here, we elucidate the prevalence of a hypertransmissible lncM1 plasmid, pKPC_NDM, co-carrying bla_KPC-2 and bla_NDM-1 genes in sequence type 1049 K_locus 5 (ST1049-KL5) KPC_NDM_CRKP isolates. Genetic and clonal relatedness analyses using pulsed-field gel electrophoresis, single nucleotide polymorphism analysis and core genome multilocus sequence typing suggested clonal dissemination of ST1049-KL5 KPC_NDM_CRKP strains in our hospital. Whole genome sequencing identified an identical 76,517 bp- bla_KPC-2 and bla_NDM-1 genes co-carrying IncM1 plasmid pKPC_NDM and a pLVPK-like hypervirulent plasmid in all ST1049-KL5 KPC_N

SUBMITTER: Liu H 

PROVIDER: S-EPMC11156905 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature