An oncogenic enhancer promotes melanoma progression via regulating ETV4 expression.

ABSTRACT:

Background

Enhancers are important gene regulatory elements that promote the expression of critical genes in development and disease. Aberrant enhancer can modulate cancer risk and activate oncogenes that lead to the occurrence of various cancers. However, the underlying mechanism of most enhancers in cancer remains unclear. Here, we aim to explore the function and mechanism of a crucial enhancer in melanoma.

Methods

Multi-omics data were applied to identify an enhancer (enh17) involved in melanoma progression. To evaluate the function of enh17, CRISPR/Cas9 technology were applied to knockout enh17 in melanoma cell line A375. RNA-seq, ChIP-seq and Hi-C data analysis integrated with luciferase reporter assay were performed to identify the potential target gene of enh17. Funct

SUBMITTER: Zhang J 

PROVIDER: S-EPMC11157841 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature