Dissecting functional roles of p53 N-terminal transactivation domains by microarray expression analysis.

ABSTRACT: The p53 protein exerts its tumor suppressive function mainly by acting as a transcription activator. Two transactivation domains (TADs) located at the amino-terminus of p53 are required for transcription activation, and the activity of TADs is tightly regulated by post-translational modifications, such as phosphorylation. We attempted to dissect the functions of the two TADs and phosphorylation within the TADs by analyzing p53 target genes induced by full-length p53 (FL-p53), N-terminally deleted p53 isoform lacking the first TAD (Delta1stTAD) and p53 carrying point mutations at all serine residues within the two TADs (TAD-S/A). By performing a comprehensive survey by employing microarray expression analysis, the induction of target genes by FL-p53, Delta1stTAD and TAD-S/A was analyzed. Al

SUBMITTER: Ohki R 

PROVIDER: S-EPMC11159457 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature