Preparation, characterization and <i>in vitro</i> evaluation of 5-fluorouracil loaded into chitosan-acacia gum nanoparticles.

ABSTRACT: Aim: In this study, we prepared, characterized and in vitro evaluated a 5-fluorouracil (5-FU)-loaded chitosan-acacia gum nanoparticles. Methods: Nanoparticles were characterized for their size, charge, morphology and encapsulation efficiency (EE%) followed by cellular investigations against HT-29 colon cancer cell line. Results: The nanoparticles exhibited a spherical morphological size with 94.42% EE%. Free 5-FU showed a fast and fully cumulative release after 6 h while 5-FU loaded into CS-AG NPs showed good entrapment and slow, prolonged 5-FU release even after 24 h. Enhanced IC₅₀ for the 5-FU loaded NPs compared with free 5-FU against HT-29 colon cancer cell line was reported with high selectivity compared with normal fibroblast cells. Conclusio

SUBMITTER: Al-Nasrawi H 

PROVIDER: S-EPMC11160445 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature