Design, Construction, and Validation of a Yeast-Displayed Chemically Expanded Antibody Library.

ABSTRACT: In vitro display technologies, exemplified by phage and yeast display, have emerged as powerful platforms for antibody discovery and engineering. However, the identification of antibodies that disrupt target functions beyond binding remains a challenge. In particular, there are very few strategies that support identification and engineering of either protein-based irreversible binders or inhibitory enzyme binders. Expanding the range of chemistries in antibody libraries has the potential to lead to efficient discovery of function-disrupting antibodies. In this work, we describe a yeast display-based platform for the discovery of chemically diversified antibodies. We constructed a billion-member antibody library that supports the presentation of a range of chemistries within antibody

SUBMITTER: Rezhdo A 

PROVIDER: S-EPMC11160716 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature