Inner limiting Membrane Peel Extends <i>In vivo</i> Calcium Imaging of Retinal Ganglion Cell Activity Beyond the Fovea in Non-Human Primate.

ABSTRACT:

Purpose

Adaptive Optics Scanning Light Ophthalmoscopy (AOSLO) paired with intravitreal injection of a viral vector coding for the calcium indicator GCaMP has enabled visualization of neuronal activity in retinal ganglion cells (RGCs) at single cell resolution in the living eye. However, the inner limiting membrane (ILM) restricts viral transduction to the fovea in humans and non-human primates (NHP), hindering both therapeutic intervention and physiological study of the retina. To address this, we explored peeling the ILM before intravitreal injection to expand calcium imaging beyond the fovea in the living primate eye.

Methods

Five eyes from Macaca fascicularis (age 3-10; n=3; 2 males, 1 female) underwent vitrectomy and ILM peel centered on the fovea prior to intravitreal d

SUBMITTER: Baez HC 

PROVIDER: S-EPMC11160754 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature