Transcriptional remodeling by OTX2 directs specification and patterning of mammalian definitive endoderm.

ABSTRACT: The molecular mechanisms that drive essential developmental patterning events in the mammalian embryo remain poorly understood. To generate a conceptual framework for gene regulatory processes during germ layer specification, we analyzed transcription factor (TF) expression kinetics around gastrulation and during in vitro differentiation. This approach identified Otx2 as a candidate regulator of definitive endoderm (DE), the precursor of all gut- derived tissues. Analysis of multipurpose degron alleles in gastruloid and directed differentiation models revealed that loss of OTX2 before or after DE specification alters the expression of core components and targets of specific cellular signaling pathways, perturbs adhesion and migration programs as well as de-represses regulator

SUBMITTER: Ee LS 

PROVIDER: S-EPMC11160813 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature