An efficient low cost means of biophysical gene transfection in primary cells.

ABSTRACT: Efficient, facile gene modification of cells has become an indispensable part of modern molecular biology. For the majority of cell lines and several primary populations, such modifications can be readily performed through a variety of methods. However, many primary cell lines such as stem cells frequently suffer from poor transfection efficiency. Though several physical approaches have been introduced to circumvent these issues, they often require expensive/specialized equipment and/or consumables, utilize substantial cell numbers and often still suffer from poor efficiency. Viral methods are capable of transducing difficult cellular populations, however such methods can be time consuming for large arrays of gene targets, present biohazard concerns, and result in expression of viral prote

SUBMITTER: Huang S 

PROVIDER: S-EPMC11161637 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature