Quantifying variation across 16S rRNA gene sequencing runs in human microbiome studies.

ABSTRACT: Recent microbiome research has incorporated a higher number of samples through more participants in a study, longitudinal studies, and metanalysis between studies. Physical limitations in a sequencing machine can result in samples spread across sequencing runs. Here we present the results of sequencing nearly 1000 16S rRNA gene sequences in fecal (stabilized and swab) and oral (swab) samples from multiple human microbiome studies and positive controls that were conducted with identical standard operating procedures. Sequencing was performed in the same center across 18 different runs. The simplified mock community showed limitations in accuracy, while precision (e.g., technical variation) was robust for the mock community and actual human positive control samples. Technical variation was t

SUBMITTER: Hoisington AJ 

PROVIDER: S-EPMC11162379 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature