Antigenic operon fragmentation and diversification mechanism in <i>Bacteroidota</i> impacts gut metagenomics and pathobionts in Crohn's disease microlesions.

ABSTRACT: Comensal Bacteroidota (Bacteroidota) and Enterobacteriacea are often linked to gut inflammation. However, the causes for variability of pro-inflammatory surface antigens that affect gut commensal/opportunistic dualism in Bacteroidota remain unclear. By using the classical lipopolysaccharide/O-antigen 'rfb operon' in Enterobacteriaceae as a surface antigen model (5-rfb-gene-cluster rfbABCDX), and a recent rfbA-typing strategy for strain classification, we characterized the integrity and conservancy of the entire rfb operon in Bacteroidota. Through exploratory analysis of complete genomes and metagenomes, we discovered that most Bacteroidota have the rfb operon fragmented into nonrandom patterns of gen

SUBMITTER: Bank NC 

PROVIDER: S-EPMC11164228 | biostudies-literature | 2024 Jan-Dec

REPOSITORIES: biostudies-literature