MARCH5 promotes hepatocellular carcinoma progression by inducing p53 ubiquitination degradation.

ABSTRACT:

Background

Human MARCH5 is a mitochondria-localized E3 ubiquitin-protein ligase that is essential for the regulation of mitochondrial dynamics. A large body of evidence suggests that imbalances in mitochondrial dynamics are strongly associated with cancer. However, the expression, biological function and prognostic significance of MARCH5 in hepatocellular carcinoma (HCC) have not been determined.

Materials and methods

The mRNA and protein expression of MARCH5 in HCC cell lines and tumor tissues was assessed by real-time quantitative PCR, Western blot analysis and immunohistochemistry. The clinical prognostic significance of MARCH5 was evaluated in 135 HCC patients. Knockdown or overexpression of MARCH5 in HCC cells was determined by in vitro cell proliferation, migration and

SUBMITTER: Cai X 

PROVIDER: S-EPMC11166841 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature