Single-cell sequencing depicts tumor architecture and empowers clinical decision in metastatic conjunctival melanoma.

ABSTRACT: Conjunctival melanoma (CoM) is a potentially devastating tumor that can lead to distant metastasis. Despite various therapeutic strategies for distant metastatic CoM, the clinical outcomes remain unfavorable. Herein, we performed single-cell RNA sequencing (scRNA-seq) of 47,017 cells obtained from normal conjunctival samples (n = 3) and conjunctival melanomas (n = 7). Notably, we noticed a higher abundance of cancer-associated fibroblasts (CAFs) in tumor microenvironment (TME), correlated with enhanced angiogenic capacity and increased VEGFR expression in distal metastatic CoM. Additionally, we observed a significant decrease in the proportion of total CD8⁺ T cells and an increase in the proportion of naive CD8⁺ T cells, contributing to a relatively quiescent immunolo

SUBMITTER: Shi H 

PROVIDER: S-EPMC11166926 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature