Multiplexed miRNA and Protein Analysis Using Digital Quantitative PCR in Microwell Arrays.

ABSTRACT: Proteins and microRNAs (miRNAs) act in tandem within biological pathways to regulate cellular functions, and their misregulation has been correlated to numerous diseases. Because of their interconnectedness, both miRNAs and proteins must be evaluated together to obtain accurate insights into the molecular pathways of pathogenesis. However, few analytical techniques can measure both classes of biomolecules in parallel from a single biological sample. Here, microfluidic digital quantitative PCR (dqPCR) was developed to simultaneously quantify miRNA and protein targets in a multiplexed assay using a single detection chemistry. This streamlined analysis was achieved by integrating base-stacking PCR and immuno-PCR in a microfluidic array platform. Analyses of let-7a (miRNA) and IL-6 (protein) w

SUBMITTER: Vanness BC 

PROVIDER: S-EPMC11168192 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature