E3 ligase FBXO22 is not significant for spermatogenesis and male fertility in mice.

ABSTRACT:

Background

F-box-only protein 22 (FBXO22), an important substrate receptor of the SKP1-Cullin-F-box (SCF) ubiquitin ligases, has been reported to be involved in many biological processes, including tumorigenesis, neurological disorders, cellular senescence, and DNA damage. However, the specific role of FBXO22 during spermatogenesis is poorly understood.

Methods

We produced Fbxo22 conditional knockout (cKO) and global knockout (KO) mice and assessed their sperm masurements using a computer-assisted sperm analysis (CASA) system. Additionally, we conducted histologic staining and immunostaining to examine the impact of Fbxo22 loss on spermatogenesis.

Results

Our results revealed that there were no notable differences in semen quality, fertility test results

SUBMITTER: Wu T 

PROVIDER: S-EPMC11170574 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature