Unique features of conventional and nonconventional introns in Euglena gracilis.

ABSTRACT:

Background

Nuclear introns in Euglenida have been understudied. This study aimed to investigate nuclear introns in Euglenida by identifying a large number of introns in Euglena gracilis (E. gracilis), including cis-spliced conventional and nonconventional introns, as well as trans-spliced outrons. We also examined the sequence characteristics of these introns.

Results

A total of 28,337 introns and 11,921 outrons were identified. Conventional and nonconventional introns have distinct splice site features; the former harbour canonical GT/C-AG splice sites, whereas the latter are capable of forming structured motifs with their terminal sequences. We observed that short introns had a preference for canonical GT-AG introns. Notably, conventional introns and outrons in E. gracilis

SUBMITTER: Gao P 

PROVIDER: S-EPMC11170887 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature