Human embryo live imaging reveals nuclear DNA shedding during blastocyst expansion and biopsy.

ABSTRACT: Proper preimplantation development is essential to assemble a blastocyst capable of implantation. Live imaging has uncovered major events driving early development in mouse embryos; yet, studies in humans have been limited by restrictions on genetic manipulation and lack of imaging approaches. We have overcome this barrier by combining fluorescent dyes with live imaging to reveal the dynamics of chromosome segregation, compaction, polarization, blastocyst formation, and hatching in the human embryo. We also show that blastocyst expansion mechanically constrains trophectoderm cells, causing nuclear budding and DNA shedding into the cytoplasm. Furthermore, cells with lower perinuclear keratin levels are more prone to undergo DNA loss. Moreover, applying trophectoderm biopsy, a mechanical pro

SUBMITTER: Domingo-Muelas A 

PROVIDER: S-EPMC11170958 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature