Single-Cell Profiling Reveals the Impact of Genetic Alterations on the Differentiation of Inflammation-Induced Murine Colon Tumors.

ABSTRACT: Genetic mutations and chronic inflammation of the colon contribute to the development of colorectal cancer (CRC). Using a murine model of inflammation-induced colon tumorigenesis, we determined how genetic mutations alter colon tumor cell differentiation. Inflammation induced by enterotoxigenic Bacteroides fragilis (ETBF) colonization of multiple intestinal neoplasia (Min^ApcΔ716/+) mice triggers loss of heterozygosity of Apc causing colon tumor formation. Here, we report that the addition of BRAF^V600E mutation (BRAF^F-V600ELgr5^{tm1(Cre/ERT2)Cle}Min^ApcΔ716/+, BLM) or knocking out Msh2 (Msh2^LoxP/LoxPVil1-creMin^ApcΔ716/+, MSH2KO) in the Min model altered colon tumor diffe

SUBMITTER: Ghobashi AH 

PROVIDER: S-EPMC11171101 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature