New Insights on Tools for Detecting β-Tubulin Polymorphisms in Trichuris trichiura Using rhAmp^TM SNP Genotyping.

ABSTRACT: Soil-transmitted helminth (STH) infections, commonly treated with benzimidazoles, are linked to resistance through single nucleotide polymorphisms (SNPs) at position 167, 198, or 200 in the β-tubulin isotype 1 gene. The aim of this study was to establish a novel genotyping assay characterized by its rapidity and specificity. This assay was designed to detect the presence of SNPs within the partial β-tubulin gene of Trichuris trichiura. This was achieved through the biallelic discrimination at codons 167, 198, and 200 by employing the competitive binding of two allele-specific forward primers. The specificity and reliability of this assay were subsequently confirmed using Trichuris samples isolated from captive primates. Furthermore, a molecular study was conducted to substant

SUBMITTER: Rivero J 

PROVIDER: S-EPMC11171370 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature