Ly6C^hi Monocytes Are Metabolically Reprogrammed in the Blood during Inflammatory Stimulation and Require Intact OxPhos for Chemotaxis and Monocyte to Macrophage Differentiation.

ABSTRACT: Acute inflammation is a rapid and dynamic process involving the recruitment and activation of multiple cell types in a coordinated and precise manner. Here, we investigate the origin and transcriptional reprogramming of monocytes using a model of acute inflammation, zymosan-induced peritonitis. Monocyte trafficking and adoptive transfer experiments confirmed that monocytes undergo rapid phenotypic change as they exit the blood and give rise to monocyte-derived macrophages that persist during the resolution of inflammation. Single-cell transcriptomics revealed significant heterogeneity within the surface marker-defined CD11b⁺Ly6G^-Ly6C^hi monocyte populations within the blood and at the site of inflammation. We show that two major transcriptional reprogramming

SUBMITTER: Purvis GSD 

PROVIDER: S-EPMC11171939 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature