Label-free fluorescence lifetime imaging for the assessment of cell viability in living tumor fragments.

ABSTRACT:

Significance

To enable non-destructive longitudinal assessment of drug agents in intact tumor tissue without the use of disruptive probes, we have designed a label-free method to quantify the health of individual tumor cells in excised tumor tissue using multiphoton fluorescence lifetime imaging microscopy (MP-FLIM).

Aim

Using murine tumor fragments which preserve the native tumor microenvironment, we seek to demonstrate signals generated by the intrinsically fluorescent metabolic co-factors nicotinamide adenine dinucleotide phosphate [NAD(P)H] and flavin adenine dinucleotide (FAD) correlate with irreversible cascades leading to cell death.

Approach

We use MP-FLIM of NAD(P)H and FAD on tissues and confirm viability using standard apoptosis and live/dead (Caspase 3/7 a

SUBMITTER: Smith JT 

PROVIDER: S-EPMC11177118 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature