OMIP-102: 50-color phenotyping of the human immune system with in-depth assessment of T cells and dendritic cells.

ABSTRACT: We report the development of an optimized 50-color spectral flow cytometry panel designed for the in-depth analysis of the immune system in human blood and tissues, with the goal of maximizing the amount of information that can be collected using currently available flow cytometry platforms. We established and tested this panel using peripheral blood mononuclear cells (PBMCs), but included CD45 to enable its future use for the analysis of human tissue samples. The panel contains lineage markers for all major immune cell subsets, and an extensive set of phenotyping markers focused on the activation and differentiation status of the T cell and dendritic cell (DC) compartment. We outline the biological insight that can be gained from the simultaneous measurement of such a large number of prot

SUBMITTER: Konecny AJ 

PROVIDER: S-EPMC11178442 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature