Proteostasis governs differential temperature sensitivity across embryonic cell types.

ABSTRACT: Embryonic development is remarkably robust, but temperature stress can degrade its ability to generate animals with invariant anatomy. Phenotypes associated with environmental stress suggest that some cell types are more sensitive to stress than others, but the basis of this sensitivity is unknown. Here, we characterize hundreds of individual zebrafish embryos under temperature stress using whole-animal single-cell RNA sequencing (RNA-seq) to identify cell types and molecular programs driving phenotypic variability. We find that temperature perturbs the normal proportions and gene expression programs of numerous cell types and also introduces asynchrony in developmental timing. The notochord is particularly sensitive to temperature, which we map to a specialized cell type: sheath cells. Th

SUBMITTER: Dorrity MW 

PROVIDER: S-EPMC11178971 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature