Pseudomonas putida Dynamics of Adaptation under Prolonged Resource Exhaustion.

ABSTRACT: Many nonsporulating bacterial species survive prolonged resource exhaustion, by entering a state termed long-term stationary phase. Here, we performed long-term stationary phase evolutionary experiments on the bacterium Pseudomonas putida, followed by whole-genome sequencing of evolved clones. We show that P. putida is able to persist and adapt genetically under long-term stationary phase. We observed an accumulation of mutations within the evolving P. putida populations. Within each population, independently evolving lineages are established early on and persist throughout the 4-month-long experiment. Mutations accumulate in a highly convergent manner, with similar loci being mutated across independently evolving populations. Across populations, mutators emerge, that due to mutations with

SUBMITTER: Gross J 

PROVIDER: S-EPMC11179108 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature