Hyperbaric oxygen treatment reveals spatiotemporal OXPHOS plasticity in the porcine heart.

ABSTRACT: Cardiomyocytes meet their high ATP demand almost exclusively by oxidative phosphorylation (OXPHOS). Adequate oxygen supply is an essential prerequisite to keep OXPHOS operational. At least two spatially distinct mitochondrial subpopulations facilitate OXPHOS in cardiomyocytes, i.e. subsarcolemmal (SSM) and interfibrillar mitochondria (IFM). Their intracellular localization below the sarcolemma or buried deep between the sarcomeres suggests different oxygen availability. Here, we studied SSM and IFM isolated from piglet hearts and found significantly lower activities of electron transport chain enzymes and F₁F_O-ATP synthase in IFM, indicative for compromised energy metabolism. To test the contribution of oxygen availability to this outcome, we ventilated piglets under

SUBMITTER: Heidler J 

PROVIDER: S-EPMC11179111 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature