Imaging quantitative changes in blood-brain barrier permeability using [¹⁸F]2-fluoro-2-deoxy-sorbitol ([¹⁸F]FDS) PET in relation to glial cell recruitment in a mouse model of endotoxemia.

ABSTRACT: The quantitative relationship between the disruption of the blood-brain barrier (BBB) and the recruitment of glial cells was explored in a mouse model of endotoxemia. [¹⁸F]2-Fluoro-2-deoxy-sorbitol ([¹⁸F]FDS) PET imaging was used as a paracellular marker for quantitative monitoring of BBB permeability after i.v injection of increasing doses of lipopolysaccharide (LPS) or vehicle (saline, n = 5). The brain distribution of [¹⁸F]FDS (V_T, mL.cm^-3) was estimated using kinetic modeling. LPS dose-dependently increased the brain V_T of [¹⁸F]FDS after injection of LPS 4 mg/kg (5.2 ± 2.4-fold, n = 4, p < 0.01) or 5 mg/kg (9.0 ± 9.1-fold, n = 4, p < 0.01) but not 3 mg/kg (p > 0.05, n = 7). In 12 individuals belo

SUBMITTER: Leterrier S 

PROVIDER: S-EPMC11179610 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature