GPX3 supports ovarian cancer tumor progression in vivo and promotes expression of GDF15.

ABSTRACT:

Objective

We previously reported that high expression of the extracellular glutathione peroxidase GPX3 is associated with poor patient outcome in ovarian serous adenocarcinomas, and that GPX3 protects ovarian cancer cells from oxidative stress in culture. Here we tested if GPX3 is necessary for tumor establishment in vivo and to identify novel downstream mediators of GPX3's pro-tumorigenic function.

Methods

GPX3 was knocked-down in ID8 ovarian cancer cells by shRNA to test the role of GPX3 in tumor establishment using a syngeneic IP xenograft model. RNA sequencing analysis was carried out in OVCAR3 cells following shRNA-mediated GPX3 knock-down to identify GPX3-dependent gene expression signatures.

Results

GPX3 knock-down abrogated clonogenicity and intraperitoneal tu

SUBMITTER: Chang C 

PROVIDER: S-EPMC11179984 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature