Systemic neutrophil degranulation and emergency granulopoiesis in patients with Clostridioides difficile infection.

ABSTRACT:

Objectives

Clostridioides difficile infection (CDI) is characterized by neutrophilia in blood, with a high leukocyte count accompanying severe infection. In this study, we characterized peripheral blood neutrophil activation and maturity in CDI by (i) developing a method to phenotype stored neutrophils for disease-related developmental alterations and (ii) assessing neutrophil-associated biomarkers.

Methods

We stored fixed leukocytes from blood collected within 24 h of diagnosis from a cohort of hospitalized patients with acute CDI. Additional study cohorts included recurrent CDI patients at time of and two months after FMT therapy and a control healthy cohort. We assessed levels of neutrophil surface markers CD66b, CD11b, CD16 and CD10 by flow cytometry. Plasma neutrophil e

SUBMITTER: Ramakrishnan G 

PROVIDER: S-EPMC11180570 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature