New uses for an old technique: live imaging on the slice organ culture to study reproductive processes†.

ABSTRACT: Reproductive processes are dynamic and involve extensive morphological remodeling and cell-cell interactions. Live imaging of organs enhances our understanding of how biological processes occur in real time. Slice culture is a type of organ culture where thick slices are collected from an organ and cultured for several days. Slice culture is a useful and easy-to-implement technique for live imaging of reproductive events at cellular resolution. Here we describe a pipeline of live imaging on slice culture to visualize the process of urethra closure in mouse embryonic penis as a proof of principle. In combination with genetic reporter mice, nuclear stains, and exposure experiments, we demonstrate the feasibility of slice culture on a reproductive organ. We also provide a step-by-step protoco

SUBMITTER: Amato CM 

PROVIDER: S-EPMC11180704 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature