Enrichment and characterization of human-associated mucin-degrading microbial consortia by sequential passage.

ABSTRACT: Mucin is a glycoprotein secreted throughout the mammalian gastrointestinal tract that can support endogenous microorganisms in the absence of complex polysaccharides. While several mucin-degrading bacteria have been identified, the interindividual differences in microbial communities capable of metabolizing this complex polymer are not well described. To determine whether community assembly on mucin is deterministic across individuals or whether taxonomically distinct but functionally similar mucin-degrading communities are selected across fecal inocula, we used a 10-day in vitro sequential batch culture fermentation from three human donors with mucin as the sole carbon source. For each donor, 16S rRNA gene amplicon sequencing was used to characterize microbial community succession, and th

SUBMITTER: Fricker AD 

PROVIDER: S-EPMC11180985 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature