A Seminested PCR Method for the Diagnosis of Invasive Fungal Infections in Combat Injured.

ABSTRACT:

Background

Among combat injured, invasive fungal infections (IFIs) result in significant morbidity. Cultures and histopathology are the primary diagnostic methods for IFIs, but they have limitations. We previously evaluated a panfungal polymerase chain reaction assay, which was 83% sensitive and 99% specific for angioinvasive IFIs. Here, we evaluated 3 less resource-intensive seminested assays targeting clinically relevant fungi in the order Mucorales and genera Aspergillus and Fusarium.

Methods

Formalin-fixed paraffin-embedded tissue specimens from a multicenter trauma IFI cohort (2009-2014) were used. Cases were US military personnel injured in Afghanistan with histopathologic IFI evidence. Controls were patients with similar injury patterns and no laboratory

SUBMITTER: Ellis GC 

PROVIDER: S-EPMC11181181 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature