Improved MRI methods to quantify retinal and choroidal blood flow applied to a model of glaucoma.

ABSTRACT:

Purpose

Blood flow (BF) of the retinal and choroidal vasculatures can be quantitatively imaged using MRI. This study sought to improve methods of data acquisition and analysis for MRI of layer-specific retinal and choroidal BF and then applied this approach to detect reduced ocular BF in a well-established mouse model of glaucoma from both eyes.

Methods

Quantitative BF magnetic resonance imaging (MRI) was performed on glaucomatous DBA/2J and normal C57BL/6J mice. Arterial spin labeling MRI was applied to image retinal and choroidal BF using custom-made dual eye coils that could image both eyes during the same scan. Statistics using data from a single eye or two eyes were compared. BF values were calculated using two approaches. The BF rate per quantity of tissue was calculat

SUBMITTER: Jiang Z 

PROVIDER: S-EPMC11182105 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature