Programmable RNA N⁶-methyladenosine editing with CRISPR/dCas13a in plants.

ABSTRACT: N⁶-methyladenonsine (m⁶A) is the most prevalent internal modification of messenger RNA (mRNA) and plays critical roles in mRNA processing and metabolism. However, perturbation of individual m⁶A modification to reveal its function and the phenotypic effects is still lacking in plants. Here, we describe the construction and characterization of programmable m⁶A editing tools by fusing the m⁶A writers, the core catalytic domain of the MTA and MTB complex, and the AlkB homologue 5 (ALKBH5) eraser, to catalytically dead Cas13a (dCas13a) to edit individual m⁶A sites on mRNAs. We demonstrated that our m⁶A editors could efficiently and specifically deposit and remove m⁶A modifications on specific RNA transcripts

SUBMITTER: Shi C 

PROVIDER: S-EPMC11182597 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature