Using a DNA mini-barcode within the ITS region to identify toxic Amanita in mushroom poisoning cases.

ABSTRACT: Mushroom poisoning contributes significantly to global foodborne diseases and related fatalities. Amanita mushrooms frequently cause such poisonings; however, identifying these toxic species is challenging due to the unavailability of fresh and intact samples. It is often necessary to analyze residues, vomitus, or stomach extracts to obtain DNA sequences for the identification of species responsible for causing food poisoning. This usually proves challenging to obtain usable DNA sequences that can be analyzed using conventional molecular biology techniques. Therefore, this study aimed to develop a DNA mini-barcoding method for the identification of Amanita species. Following the evaluation and optimization of universal primers for DNA mini-barcoding in Amanita mushrooms, we found that the

SUBMITTER: Xing RR 

PROVIDER: S-EPMC11182838 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature