Structural and biophysical insights into targeting of claudin-4 by a synthetic antibody fragment.

ABSTRACT: Claudins are a 27-member family of ~25 kDa membrane proteins that integrate into tight junctions to form molecular barriers at the paracellular spaces between endothelial and epithelial cells. As the backbone of tight junction structure and function, claudins are attractive targets for modulating tissue permeability to deliver drugs or treat disease. However, structures of claudins are limited due to their small sizes and physicochemical properties-these traits also make therapy development a challenge. Here we report the development of a synthetic antibody fragment (sFab) that binds human claudin-4 and the determination of a high-resolution structure of it bound to claudin-4/enterotoxin complexes using cryogenic electron microscopy. Structural and biophysical results reveal this sFabs mec

SUBMITTER: Erramilli SK 

PROVIDER: S-EPMC11183071 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature