Mapping of afferent and efferent connections of phenylethanolamine N-methyltransferase-expressing neurons in the nucleus tractus solitarii.

ABSTRACT:

Objective

Phenylethanolamine N-methyltransferase (PNMT)-expressing neurons in the nucleus tractus solitarii (NTS) contribute to the regulation of autonomic functions. However, the neural circuits linking these neurons to other brain regions remain unclear. This study aims to investigate the connectivity mechanisms of the PNMT-expressing neurons in the NTS (NTS^PNMT neurons).

Methods

The methodologies employed in this study included a modified rabies virus-based retrograde neural tracing technique, conventional viral anterograde tracing, and immunohistochemical staining procedures.

Results

A total of 43 upstream nuclei projecting to NTS^PNMT neurons were identified, spanning several key brain regions including the medulla oblongata, pons, midbrain,

SUBMITTER: Zhu M 

PROVIDER: S-EPMC11183208 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature