Defining mesenchymal stem/stromal cell-induced myeloid-derived suppressor cells using single-cell transcriptomics.

ABSTRACT: Mesenchymal stem/stromal cells (MSCs) modulate the immune response through interactions with innate immune cells. We previously demonstrated that MSCs alleviate ocular autoimmune inflammation by directing bone marrow cell differentiation from pro-inflammatory CD11b^hiLy6C^hiLy6G^lo cells into immunosuppressive CD11b^midLy6C^midLy6G^lo cells. Herein, we analyzed MSC-induced CD11b^midLy6C^mid cells using single-cell RNA sequencing and compared them with CD11b^hiLy6C^hi cells. Our investigation revealed seven distinct immune cell types including myeloid-derived suppressor cells (MDSCs) in the CD11b^midLy6C^mid cells, while CD11b^hiLy6C^hi cells included m

SUBMITTER: Lee HJ 

PROVIDER: S-EPMC11184332 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature