C. elegans LIN-66 mediates EIF-3/eIF3-dependent protein translation via a cold-shock domain.

ABSTRACT: Protein translation initiation is a conserved process involving many proteins acting in concert. The 13 subunit eukaryotic initiation factor 3 (eIF3) complex is essential for assembly of the pre-initiation complex that scans mRNA and positions ribosome at the initiation codon. We previously reported that a gain-of-function (gf) mutation affecting the G subunit of the Caenorhabditis elegans eIF3 complex, eif-3.g(gf), selectively modulates protein translation in the ventral cord cholinergic motor neurons. Here, through unbiased genetic suppressor screening, we identified that the gene lin-66 mediates eif-3.g(gf)-dependent protein translation in motor neurons. LIN-66 is composed largely of low-complexity amino acid sequences with unknown functional domains.

SUBMITTER: Blazie SM 

PROVIDER: S-EPMC11184513 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature