Development of a Stable Peptide-Major Histocompatibility Complex (MHC) via Sortase and Click Chemistry.

ABSTRACT: T cells play a crucial role in antitumor immune responses and the clearance of infected cells. They identify their targets through the binding of T-cell receptors (TCRs) to peptide-major histocompatibility complex (pMHC) molecules present in cancer cells, infected cells, and antigen-presenting cells. This interaction is often weak, requiring multimeric pMHC molecules to enhance the avidity for identifying antigen-specific T cells. Current exchangeable pMHC-I tetramerization methods may overlook TCRs recognizing less stable yet immunogenic peptides. In vivo applications targeting antigen-specific T cells demand the genetic synthesis of a pMHC fusion for each unique peptide antigen, which poses a significant challenge. To address these challenges, we developed a sortase and click chem

SUBMITTER: Uslu SC 

PROVIDER: S-EPMC11184609 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature