Reprogramming of 3D genome structure underlying HSPC development in zebrafish.

ABSTRACT:

Background

Development of hematopoietic stem and progenitor cells (HSPC) is a multi-staged complex process that conserved between zebrafish and mammals. Understanding the mechanism underlying HSPC development is a holy grail of hematopoietic biology, which is helpful for HSPC clinical application. Chromatin conformation plays important roles in transcriptional regulation and cell fate decision; however, its dynamic and role in HSPC development is poorly investigated.

Methods

We performed chromatin structure and multi-omics dissection across different stages of HSPC developmental trajectory in zebrafish for the first time, including Hi-C, RNA-seq, ATAC-seq, H3K4me3 and H3K27ac ChIP-seq.

Results

The chromatin organization of zebrafish HSPC resemble mammalian cells with

SUBMITTER: He M 

PROVIDER: S-EPMC11184745 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature