Combined HRAS and NRAS ablation induces a RASopathy phenotype in mice.

ABSTRACT:

Background

HRAS^KO/NRAS^KO double knockout mice exhibit exceedingly high rates of perinatal lethality due to respiratory failure caused by a significant lung maturation delay. The few animals that reach adulthood have a normal lifespan, but present areas of atelectasis mixed with patches of emphysema and normal tissue in the lung.

Methods

Eight double knockout and eight control mice were analyzed using micro-X-ray computerized tomography and a Small Animal Physiological Monitoring system. Tissues and samples from these mice were analyzed using standard histological and Molecular Biology methods and the significance of the results analyzed using a Student´s T-test.

Results

The very few double knockout mice surviving up to adulthood display clear cra

SUBMITTER: Fuentes-Mateos R 

PROVIDER: S-EPMC11184836 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature