Single-cell RNA transcriptomic reveal the mechanism of MSC derived small extracellular vesicles against DKD fibrosis.

ABSTRACT: Diabetic kidney disease (DKD), a chronic kidney disease, is characterized by progressive fibrosis caused due to persistent hyperglycemia. The development of fibrosis in DKD determines the patient prognosis, but no particularly effective treatment. Here, small extracellular vesicles derived from mesenchymal stem cells (MSC-sEV) have been used to treat DKD fibrosis. Single-cell RNA sequencing was used to analyze 27,424 cells of the kidney, we have found that a novel fibrosis-associated TGF-β₁⁺Arg1⁺ macrophage subpopulation, which expanded and polarized in DKD and was noted to be profibrogenic. Additionally, Actin⁺Col4a5⁺ mesangial cells in DKD differentiated into myofibroblasts. Multilineage ligand-receptor and cell-communication analysi

SUBMITTER: Ji C 

PROVIDER: S-EPMC11184851 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature