Infiltrating CD8⁺ T cells exacerbate Alzheimer's disease pathology in a 3D human neuroimmune axis model.

ABSTRACT: Brain infiltration of peripheral immune cells and their interactions with brain-resident cells may contribute to Alzheimer's disease (AD) pathology. To examine these interactions, in the present study we developed a three-dimensional human neuroimmune axis model comprising stem cell-derived neurons, astrocytes and microglia, together with peripheral immune cells. We observed an increase in the number of T cells (but not B cells) and monocytes selectively infiltrating into AD relative to control cultures. Infiltration of CD8⁺ T cells into AD cultures led to increased microglial activation, neuroinflammation and neurodegeneration. Using single-cell RNA-sequencing, we identified that infiltration of T cells into AD cultures led to induction of interferon-γ and neuroinflammatory pat

SUBMITTER: Jorfi M 

PROVIDER: S-EPMC11184920 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature