KMT2A and chronic inflammation as potential drivers of sporadic parathyroid adenoma.

ABSTRACT:

Background

Sporadic parathyroid adenoma (PA) is the most common cause of hyperparathyroidism, yet the mechanisms involved in its pathogenesis remain incompletely understood.

Methods

Surgically removed PA samples, along with normal parathyroid gland (PG) tissues that were incidentally dissected during total thyroidectomy, were analysed using single-cell RNA-sequencing with the 10× Genomics Chromium Droplet platform and Cell Ranger software. Gene set variation analysis was conducted to characterise hallmark pathway gene signatures, and single-cell regulatory network inference and clustering were utilised to analyse transcription factor regulons. Immunohistochemistry and immunofluorescence were performed to validate cellular components of PA tissues. siRNA knockdown and gene ov

SUBMITTER: Xu Q 

PROVIDER: S-EPMC11185127 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature