Heterochromatic 3D genome organization is directed by HP1a- and H3K9-dependent and independent mechanisms.

ABSTRACT: Whether and how histone post-translational modifications and the proteins that bind them drive 3D genome organization remains unanswered. Here, we evaluate the contribution of H3K9-methylated constitutive heterochromatin to 3D genome organization in Drosophila tissues. We find that the predominant organizational feature of wild-type tissues is the segregation of euchromatic chromosome arms from heterochromatic pericentromeres. Reciprocal perturbation of HP1a⋅H3K9me binding, using a point mutation in the HP1a chromodomain or replacement of the replication-dependent histone H3 with H3^K9R mutant histones, revealed that HP1a binding to methylated H3K9 in constitutive heterochromatin is required to limit contact frequency between pericentromeres and chromosome arms and regulate the d

SUBMITTER: Stutzman AV 

PROVIDER: S-EPMC11185254 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature