The COMBO window: A chronic cranial implant for multiscale circuit interrogation in mice.

ABSTRACT: Neuroscientists studying the neural correlates of mouse behavior often lack access to the brain-wide activity patterns elicited during a specific task of interest. Fortunately, large-scale imaging is becoming increasingly accessible thanks to modalities such as Ca2+ imaging and functional ultrasound (fUS). However, these and other techniques often involve challenging cranial window procedures and are difficult to combine with other neuroscience tools. We address this need with an open-source 3D-printable cranial implant-the COMBO (ChrOnic Multimodal imaging and Behavioral Observation) window. The COMBO window enables chronic imaging of large portions of the brain in head-fixed mice while preserving orofacial movements. We validate the COMBO window stability using both brain-wide fUS and mu

SUBMITTER: Edelman BJ 

PROVIDER: S-EPMC11185485 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature