Decoding chromosome organization using CheC-PLS: chromosome conformation by proximity labeling and long-read sequencing.

ABSTRACT: Genomic approaches have provided detailed insight into chromosome architecture. However, commonly deployed techniques do not preserve connectivity-based information, leaving large-scale genome organization poorly characterized. Here, we developed CheC-PLS: a proximity-labeling technique that indelibly marks, and then decodes, protein-associated sites. CheC-PLS tethers dam methyltransferase to a protein of interest, followed by Nanopore sequencing to identify methylated bases - indicative of in vivo proximity - along reads >100kb. As proof-of-concept we analyzed, in budding yeast, a cohesin-based meiotic backbone that organizes chromatin into an array of loops. Our data recapitulates previously obtained association patterns, and, importantly, exposes variability between cells. Single

SUBMITTER: Xu K 

PROVIDER: S-EPMC11185558 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature