PT-seq: A method for metagenomic analysis of phosphorothioate epigenetics in complex microbial communities.

ABSTRACT: Among dozens of known epigenetic marks, naturally occurring phosphorothioate (PT) DNA modifications are unique in replacing a non-bridging phosphate oxygen with redox-active sulfur and function in prokaryotic restriction-modification and transcriptional regulation. Interest in PTs has grown due to the widespread distribution of the dnd, ssp, and brx genes among bacteria and archaea, as well as the discovery of PTs in 5-10% of gut microbes. Efforts to map PTs in complex microbiomes using existing next-generation and direct sequencing technologies have failed due to poor sensitivity. Here we developed PT-seq as a high-sensitivity method to quantitatively map PTs across genomes and metagenomically identify PT-containing microbes in complex genomic mixtures. Like other methods fo

SUBMITTER: Yuan Y 

PROVIDER: S-EPMC11185561 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature